暑期特辑 | 我们读过的书走过的路，终将化作我们的视野
暑期特辑 | 这颗明亮的星，仍需更猛烈的火来点燃它，我也是如此
Internship in the New Zealand -- Chinese Kiwi Fruit Joint Test Base
中英图文by Mary Wu
In order to enrich my extremely poor social practice experience, this summer vacation, my classmates and I finally seized the opportunity to start a 7-day internship in "New Zealand -- Chinese Kiwi Fruit Joint Test Base", Science City of Tianfu new district.
As a state-level comprehensive functional area that president Xi pays close attention to, Tianfu new district has been occupied by six major industries including electronic information, automobile manufacturing, new energy, new materials, bio-medicine and finance since 2012. Seven years later, it has become an important node in the construction of "One Belt And One Road" and the development of the Yangtze river economic belt. The kiwi laboratory located in the north side of Xinglong Lake was set up in the boom of new district development.
The focus of my internship was on the analysis of the pathological pest and pathogen culture of kiwi fruit, and I had the opportunity to go into the pathological analysis room, nucleic acid extraction room, PCR room and other laboratories to study the character and genes of samples. Here, the researchers analyze the leaves and branches of wild kiwifruit from Jiuzhaigou, Dujiangyan and regions along the Yangtze river to find pathogens (usually bacteria or fungi) and collect them in the Kiwifruit Gene Bank.
The first few days were the first purification of the bacteria. We sterilized the collected kiwi leaves and sealed them in the sterilizing laboratory bag. After adding a small amount of normal saline, the leaves were pounded and crushed until the juice spilled, and then let stand for 4 hours. A little supernatant was dipped in with a biological marker and spread on the petri dish.
Because the growth cycle of bacteria was fast, large visible colonies can be produced in a single night. Since most of the leaves have more than one pathogen, secondary and tertiary purification is very crucial. The principle is simple: select individual similar colonies from the first purified diverse bacteria and reproduce them until the colonies grown in the petri dish are identical in color and state.
When a single, purified strain was obtained, it is an important step to preserve the isolated strain for a long time. In just one day, we needed to preserve more than 300 samples in normal saline, glycerin, and potassium culture solution, and ensured their absolute purity.
Because of the need to avoid all foreign bacterium contamination, the operation of the instrument, the angle of the body, and even the movement of the hands are placed in strict requirements, which is undoubtedly a very difficult test for the experimenter.
Such basic survey sampling is a required course for basic laboratory researchers. In the research process of more than 10 years, the kiwi laboratory has collected tens of thousands of branch and leaf samples, and formed more than 2 million resource evaluation data. This is essential for understanding the pathologic solutions of all kiwi varieties.
Although the basic sampling and observation work may seem tedious and simple to researchers, I finally realized the importance of it to those who engaged in genetic engineering and crossbreeding in the future. In every scientific research work, repetitive and boring basic research in the initial stage is an important step to understand the development law of things and clarify the growth needs of things.
It takes at least five years to develop new kiwi varieties, from screening, hybridization to results. Due to the lack of national scientific research funds, more and more "high platform, low threshold problems" start to appear. Graduates are more willing to work in foreign companies, sino-foreign joint ventures and large pharmaceutical enterprises with high salary, and are dismissive of domestic independent scientific research institutions with low income and long working cycle.
I wish the state would allocate more funds to research enterprises, so that more talented and thoughtful young graduates could devote themselves to independent and self-sufficient national research projects, making more contributions to the prosperity of the technology and innovation of China.